After appropriately preparing the biopsy site, introduce the bone marrow needle perpendicular to the surface of the bone, holding the needle firmly in the palm of your hand.

Using gentle, but firm pressure, advance the needle tip through the periosteum, into the cortex, by rotating in an alternating clockwise-counterclockwise motion.

Remove the stylette from the needle cannula by rotating upper section 90º and pulling straight out.

Continue advancing the needle cannula forward 2 to 3 centimetres, while rotating in an alternating clockwise-counterclockwise motion, into the bone marrow. Decreased resistance indicates entrance into the bone marrow cavity.

Insert the marked obturator/probe without force, to check the sample length in the needle lumen. Sample length is indicated by referencing the position of the “mark” to the cannula hub. Remove the probe.